Insulin resistance is a crucial factor correlating with and contributing to obesity, the metabolic syndrome, and diabetes. Understanding of mechanisms of insulin resistance is vital for prevention and treatment of these metabolic disorders. Recently, a novel signaling class of lipids, branched fatty acid esters of hydroxyl fatty acids (FAHFAs) has been identified. We developed a chemical analytical procedure for a targeted metabolomics assay for quantification of different molecular lipids of the FAHFAs family in human plasma (DZD-funded project).
Role of FAHFAS in mediation of mechanisms of insuline resistance
The aim of PhD project proposal is to adopt the assay to biological matrices and to perform a validation and measurements in a human cohort with insulin resistance phenotype.
The outcome of this project will be: 1) a biologically validated, reliable analytical assay, which will be an indispensable tool for both, the metabolic phenotyping of pre-diabetes but as well for the analysis of mechanisms of metabolic diseases in human and 2) analyses of metabolic pathways in humans at different insuline progression stages. The candidate will work in a laboratory equipped with state-of-the art robotics for liquit handling and mass spectormetry for analytics. Further, the advanced biostatistical procedures will be co-developed during the PhD to infere metabolomic signatures associated with insuline resistance.
Prerequisites for the project: education as biochemists or chemists and knowledge in biostatistics, preferentially with a Master’s degree in one of the two disciplines.